Coding
Part:BBa_M45122:Design
Designed by: Sherissa Ward Group: UtahState_BE5930_-_S14 (2014-04-15)
Autolysin N-acetylmuramoyl-L-alanine amidase (cwlB) gene for E coli, complete cds
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This gene sequence required one base pair mutation after being codon optimized for E. coli
Source
The gene sequence for the cwlB amidase was taken from Bacillus subtilis on the NCBI database